Rojo-Bustamante_2020_Biomolecules_10_

Reference

Title : CB2 Receptors and Neuron-Glia Interactions Modulate Neurotoxicity Generated by MAGL Inhibition - Rojo-Bustamante_2020_Biomolecules_10_
Author(s) : Rojo-Bustamante E , nigo-Marco I , Abellanas MA , Vinueza-Gavilanes R , Baltanas A , Luquin E , Arrasate M , Aymerich MS
Ref : Biomolecules , 10 : , 2020
Abstract :

Monoacylglycerol lipase inhibition (MAGL) has emerged as an interesting therapeutic target for neurodegenerative disease treatment due to its ability to modulate the endocannabinoid system and to prevent the production of proinflammatory mediators. To obtain a beneficial response, it is necessary to understand how this inhibition affects the neuron-glia crosstalk and neuron viability. In this study, the effect of MAGL inhibition by KML29 was evaluated in two types of rat cortical primary cultures; mixed cultures, including neuron and glial cells, and neuron-enriched cultures. The risk of neuronal death was estimated by longitudinal survival analysis. The spontaneous neuronal risk of death in culture was higher in the absence of glial cells, a process that was enhanced by KML29 addition. In contrast, neuronal survival was not compromised by MAGL inhibition in the presence of glial cells. Blockade of cannabinoid type 2 (CB2) receptors expressed mainly by microglial cells did not affect the spontaneous neuronal death risk but decreased neuronal survival when KML29 was added. Modulation of cannabinoid type 1 (CB1) receptors did not affect neuronal survival. Our results show that neuron-glia interactions are essential for neuronal survival. CB2 receptors play a key role in these protective interactions when neurons are exposed to toxic conditions.

PubMedSearch : Rojo-Bustamante_2020_Biomolecules_10_
PubMedID: 32824740

Related information

Citations formats

Rojo-Bustamante E, nigo-Marco I, Abellanas MA, Vinueza-Gavilanes R, Baltanas A, Luquin E, Arrasate M, Aymerich MS (2020)
CB2 Receptors and Neuron-Glia Interactions Modulate Neurotoxicity Generated by MAGL Inhibition
Biomolecules 10 :

Rojo-Bustamante E, nigo-Marco I, Abellanas MA, Vinueza-Gavilanes R, Baltanas A, Luquin E, Arrasate M, Aymerich MS (2020)
Biomolecules 10 :