Roll_1993_Brain.Res_604_260

Reference

Title : The chronic infusion of nicotine into the developing chick embryo does not alter the density of (-)-[3H]nicotine-binding sites or vestibular function - Roll_1993_Brain.Res_604_260
Author(s) : Roll RL , Jones TA , Benowitz NL , Morley BJ
Ref : Brain Research , 604 :260 , 1993
Abstract :

(-)-Nicotine (1.2 mg/day) or saline was infused into chick embryos (Gallus domesticus) for 10 days beginning 12 h beyond the eight day of incubation (E8 + 12 h). Twelve h beyond the eighteenth day of incubation (E18 + 12 h), the eggs were opened to access the embryos and subcutaneous skull electrodes placed. Short latency vestibular response thresholds and input/output functions were determined to assess neurophysiological consequences of chronic nicotine administration. Samples of serum and extraembryonic (amniotic and albumen) fluid were analyzed by gas chromatography-mass spectrometry to determine the levels of nicotine and its major metabolite, cotinine. The brains were removed and divided into diencephalon and mesencephalon and the density of (-)-[3H]nicotine binding sites in each brain area was measured. Nicotine and cotinine were found in the serum and extraembryonic fluid, but nicotinic receptors were not up-regulated in the brains of animals infused with nicotine in comparison to controls. Vestibular response thresholds also did not differ between nicotine-treated and control animals.

PubMedSearch : Roll_1993_Brain.Res_604_260
PubMedID: 8457853

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Citations formats

Roll RL, Jones TA, Benowitz NL, Morley BJ (1993)
The chronic infusion of nicotine into the developing chick embryo does not alter the density of (-)-[3H]nicotine-binding sites or vestibular function
Brain Research 604 :260

Roll RL, Jones TA, Benowitz NL, Morley BJ (1993)
Brain Research 604 :260