| Title : Proteomic identification of palmitoylated proteins - Roth_2006_Methods_40_135 |
| Author(s) : Roth AF , Wan J , Green WN , Yates JR , Davis NG |
| Ref : Methods , 40 :135 , 2006 |
| Abstract : A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae. |
| ESTHER : Roth_2006_Methods_40_135 |
| PubMedSearch : Roth_2006_Methods_40_135 |
| PubMedID: 17012025 |
Roth AF, Wan J, Green WN, Yates JR, Davis NG (2006)
Proteomic identification of palmitoylated proteins
Methods
40 :135
Roth AF, Wan J, Green WN, Yates JR, Davis NG (2006)
Methods
40 :135