Roth_2014_Appl.Microbiol.Biotechnol_98_7815

Reference

Title : Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca - Roth_2014_Appl.Microbiol.Biotechnol_98_7815
Author(s) : Roth C , Wei R , Oeser T , Then J , Follner C , Zimmermann W , Strater N
Ref : Applied Microbiology & Biotechnology , 98 :7815 , 2014
Abstract : Bacterial cutinases are promising catalysts for the modification and degradation of the widely used plastic polyethylene terephthalate (PET). The improvement of the enzyme for industrial purposes is limited due to the lack of structural information for cutinases of bacterial origin. We have crystallized and structurally characterized a cutinase from Thermobifida fusca KW3 (TfCut2) in free as well as in inhibitor-bound form. Together with our analysis of the thermal stability and modelling studies, we suggest possible reasons for the outstanding thermostability in comparison to the less thermostable homolog from Thermobifida alba AHK119 and propose a model for the binding of the enzyme towards its polymeric substrate. The TfCut2 structure is the basis for the rational design of catalytically more efficient enzyme variants for the hydrolysis of PET and other synthetic polyesters.
ESTHER : Roth_2014_Appl.Microbiol.Biotechnol_98_7815
PubMedSearch : Roth_2014_Appl.Microbiol.Biotechnol_98_7815
PubMedID: 24728714
Gene_locus related to this paper: thefu-q6a0i4

Related information

Gene_locus related to this paper: thefu-q6a0i4

Citations formats

Roth C, Wei R, Oeser T, Then J, Follner C, Zimmermann W, Strater N (2014)
Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca
Applied Microbiology & Biotechnology 98 :7815

Roth C, Wei R, Oeser T, Then J, Follner C, Zimmermann W, Strater N (2014)
Applied Microbiology & Biotechnology 98 :7815