Royter_2009_Extremophiles_13_769

Reference

Title : Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis - Royter_2009_Extremophiles_13_769
Author(s) : Royter M , Schmidt M , Elend C , Hobenreich H , Schafer T , Bornscheuer UT , Antranikian G
Ref : Extremophiles , 13 :769 , 2009
Abstract :

Two novel genes encoding for heat and solvent stable lipases from strictly anaerobic extreme thermophilic bacteria Thermoanaerobacter thermohydrosulfuricus (LipTth) and Caldanaerobacter subterraneus subsp. tengcongensis (LipCst) were successfully cloned and expressed in E. coli. Recombinant proteins were purified to homogeneity by heat precipitation, hydrophobic interaction, and gel filtration chromatography. Unlike the enzymes from mesophile counterparts, enzymatic activity was measured at a broad temperature and pH range, between 40 and 90 degrees C and between pH 6.5 and 10; the half-life of the enzymes at 75 degrees C and pH 8.0 was 48 h. Inhibition was observed with 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride and phenylmethylsulfonylfluorid indicating that serine and thiol groups play a role in the active site of the enzymes. Gene sequence comparisons indicated very low identity to already described lipases from mesophilic and psychrophilic microorganisms. By optimal cultivation of E. coli Tuner (DE3) cells in 2-l bioreactors, a massive production of the recombinant lipases was achieved (53-2200 U/l) Unlike known lipases, the purified robust proteins are resistant against a large number of organic solvents (up to 99%) and detergents, and show activity toward a broad range of substrates, including triacylglycerols, monoacylglycerols, esters of secondary alcohols, and p-nitrophenyl esters. Furthermore, the enzyme from T. thermohydrosulfuricus is suitable for the production of optically pure compounds since it is highly S-stereoselective toward esters of secondary alcohols. The observed E values for but-3-yn-2-ol butyrate and but-3-yn-2-ol acetate of 21 and 16, respectively, make these enzymes ideal candidates for kinetic resolution of synthetically useful compounds.

PubMedSearch : Royter_2009_Extremophiles_13_769
PubMedID: 19579003
Gene_locus related to this paper: theet-q3ch51 , thete-TTE1809

Related information

Substrate Trihexanoin
Gene_locus theet-q3ch51    thete-TTE1809

Citations formats

Royter M, Schmidt M, Elend C, Hobenreich H, Schafer T, Bornscheuer UT, Antranikian G (2009)
Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis
Extremophiles 13 :769

Royter M, Schmidt M, Elend C, Hobenreich H, Schafer T, Bornscheuer UT, Antranikian G (2009)
Extremophiles 13 :769