Sakurada_2010_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_878_1414

Reference

Title : Liquid chromatography-tandem mass spectrometry method for determination of the pyridinium aldoxime 4-PAO in brain, liver, lung, and kidney - Sakurada_2010_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_878_1414
Author(s) : Sakurada K , Ohta H
Ref : Journal of Chromatography B Analyt Technol Biomed Life Sciences , 878 :1414 , 2010
Abstract :

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was validated and applied to the in vitro determination of 4-[(hydroxyimino)methyl]-1-octylpyridinium cation (4-PAO), which can penetrate the blood-brain barrier and reactivate acetylcholinesterase (AChE) inhibited by alkylphosphonate in the brain, liver, lung, and kidney. The limit of detection (LOD) was 0.235 microg cation/g wet weight, and the quantification range and linearity of the calibration curve extended over a range of 0.470-941 microg cation/g wet weight. For the proof of applicability, when 4-PAO was administrated intravenously via the rat tail vein at 10% LD(50), we were able to quantify the 4-PAO concentration in the tissues: brain 7.60+/-1.32 microg cation/g wet weight (mean+/-SD, n=5), liver 26.8+/-2.82 microg cation/g, lung 76.4+/-24.9mugcation/g, and kidney 638+/-266 microg cation/g. In addition, the methods for 4-[(hydroxyimino)methyl]-1-decylpyridinium bromide (4-PAD) and 4-[(hydroxyimino)methyl]-1-(2-phenylethyl) pyridinium bromide (4-PAPE) were partly validated referring to the findings of the 4-PAO full validation. Thus, the LC-MS/MS method described in this study can be useful for quantification of pyridinium aldoxime methiodide (PAM)-type oximes in biological samples.

PubMedSearch : Sakurada_2010_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_878_1414
PubMedID: 20138594

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Citations formats

Sakurada K, Ohta H (2010)
Liquid chromatography-tandem mass spectrometry method for determination of the pyridinium aldoxime 4-PAO in brain, liver, lung, and kidney
Journal of Chromatography B Analyt Technol Biomed Life Sciences 878 :1414

Sakurada K, Ohta H (2010)
Journal of Chromatography B Analyt Technol Biomed Life Sciences 878 :1414