Salamone_2011_J.Neuroimmunol_236_47

Reference

Title : Cholinergic modulation of dendritic cell function - Salamone_2011_J.Neuroimmunol_236_47
Author(s) : Salamone G , Lombardi G , Gori S , Nahmod K , Jancic C , Amaral MM , Vermeulen M , Espanol A , Sales ME , Geffner J
Ref : Journal of Neuroimmunology , 236 :47 , 2011
Abstract :

Dendritic cells (DCs) are highly specialized antigen-presenting cells with a unique ability to activate resting T lymphocytes. Acetylcholine (ACh) is the primary parasympathetic neurotransmitter and also a non-neural paracrine factor produced by different cells. Here, we analyzed the expression of the cholinergic system in DCs. We found that DCs express the muscarinic receptors M(3), M(4) and M(5), as well as the enzymes responsible for the synthesis and degradation of ACh, choline acetyltransferase (ChAT) and acetylcholinesterase (AChE), respectively. Differentiation of DCs in the presence of the cholinergic agonist carbachol, the synthetic analog of ACh, resulted in an increased expression of HLA-DR and CD86 and the stimulation of TNF-alpha and IL-8 production. All these effects were prevented by atropine, a muscarinic ACh receptor (mAChR) antagonist. Carbachol, was also able to modulate the function of DCs when added after the differentiation is accomplished; it increased the expression of HLA-DR, improved the T cell priming ability of DCs, and stimulated the production of TNF-alpha but not IL-12 or IL-10. By contrast, carbachol significantly inhibited the stimulation of HLA-DR expression and the enhancement in the T cell priming ability of DCs triggered by LPS. Interestingly, the TNF-alpha antagonist etanercept completely prevented the increased expression of HLA-DR induced by carbachol, suggesting that it promotes the phenotypic maturation of DCs by stimulating the production of TNF-alpha. ACh induced similar effects than carbachol; it stimulated the expression of HLA-DR and the production of TNF-alpha, while inhibiting the stimulation of HLA-DR expression and IL-12 production triggered by LPS. Similarly, neostigmine, an inhibitor of AChE, also stimulated the expression of HLA-DR and the production of TNF-alpha by DCs while inhibiting the production of TNF-alpha and IL-12 triggered by LPS. These results support the existence of an autocrine/paracrine loop through which ACh modulates the function of DCs.

PubMedSearch : Salamone_2011_J.Neuroimmunol_236_47
PubMedID: 21665296

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Citations formats

Salamone G, Lombardi G, Gori S, Nahmod K, Jancic C, Amaral MM, Vermeulen M, Espanol A, Sales ME, Geffner J (2011)
Cholinergic modulation of dendritic cell function
Journal of Neuroimmunology 236 :47

Salamone G, Lombardi G, Gori S, Nahmod K, Jancic C, Amaral MM, Vermeulen M, Espanol A, Sales ME, Geffner J (2011)
Journal of Neuroimmunology 236 :47