Saleem_2022_Chem.Biodivers__

Leptadenia pyrotechnica (Forssk.) Decne is growing in Cholistan desert, and is known for its laxative, analgesic, anabolic and astringent, antioxidant, anti-inflammatory, antibacterial, antitumor, hypolipidemic & antiatherosclerotic properties. The present study disclosed the metabolic picture of L. pyrotechnica and validates its folk uses. LP-H fraction constitute 25.79+/-0.11 mgGAE/g extract and 20.64+/-0.33 mgRE/g extract of phenolic and flavonoid content, respectively, followed by LP-E (23.15+/-0.33 mgGAE/g extract and 19.40+/-0.13 mgRE/g extract), however, LP-E exhibited the highest free radical scavenging (DPPH: 21.05+/-0.45mgTE/g and ABTS: 68.12+/-0.53 mgTE/g) and metal reducing (FRAP: 44.93+/-1.66, CUPRAC: 117.42+/-1.28 mgTE/g, respectively) activities. The total antioxidant capacity in Phosphomolybdenum assay (1.52+/-0.14 mmolTE/g) and ferrous ion chelating (11.57+/-0.29 mgEDTAE/g) activities were observed highest for LP-H extract. In cholinesterases inhibitory assays, the LP-E and LP-W extracts exhibited inhibitory values as 2.43 and 2.40 +/- 0.07 mgGALAE/g extract, respectively against AChE, while against BChE the LP-H displayed the highest value as 5.98+/-0.44mgGALAE/g extract. The LP-H fraction also showed the highest inhibition potential (7.72+/-0.14mmol ACAE/g and 0.55+/-0.01mmol ACAE/g, respectively) against a-glucosidase and alpha-amylase enzymes, while, in tyrosinase inhibitory assay, all the fractions exhibited significant activities in the range of 59.35+/-0.29 to 55.18+/-0.49 mgKAE/g extract. RP-UHPLC-MS analysis of LP-M disclosed the presence of 57 metabolites of various classes. A multivariate analysis and molecular docking study was also carried out to establish relationships between the metabolites and the biological activities, which finally validate the use of L. pyrotechnica as herbal medicine or component nutraceutical, food and cosmetic industry.