Title : Modulation of lipase hydrolysis and synthesis reactions using carbohydrates - Sanchez-Montero_1991_Biochim.Biophys.Acta_1078_345 |
Author(s) : Sanchez-Montero JM , Hamon V , Thomas D , Legoy MD |
Ref : Biochimica & Biophysica Acta , 1078 :345 , 1991 |
Abstract :
A novel method for modulation of lipase hydrolysis and synthesis lipase was investigated by using carbohydrates in the microenvironment of the Candida rugosa enzyme. The influence of the addition of different sugars to the previously dialysed enzyme was tested on the two reactions. Rates of hydrolysis were lowered by using dialysed enzyme but were increased after sugar addition, regardless of the identity of the added sugar. In contrast, synthesis reaction rates depended on the nature of the carbohydrate. Rates were increased by adding lactose, which is not a water activity depressor, but were lowered by adding fructose, glucose, sucrose or sorbitol, which are all water activity depressors. |
PubMedSearch : Sanchez-Montero_1991_Biochim.Biophys.Acta_1078_345 |
PubMedID: 1859825 |
Sanchez-Montero JM, Hamon V, Thomas D, Legoy MD (1991)
Modulation of lipase hydrolysis and synthesis reactions using carbohydrates
Biochimica & Biophysica Acta
1078 :345
Sanchez-Montero JM, Hamon V, Thomas D, Legoy MD (1991)
Biochimica & Biophysica Acta
1078 :345