Sani_2019_J.Adv.Res_16_87

Reference

Title : A standardised Andrographis paniculata Burm. Nees aqueous extract prevents Lipopolysaccharide-induced cognitive deficits through suppression of inflammatory cytokines and oxidative stress mediators - Sani_2019_J.Adv.Res_16_87
Author(s) : Sani D , Khatab NIO , Kirby BP , Yong A , Hasan S , Basri H , Stanslas J
Ref : J Adv Res , 16 :87 , 2019
Abstract :

Substantial evidence has shown that most cases of memory impairment are associated with increased neuroinflammation and oxidative stress. In this study, the potential of a standardised Andrographis paniculata aqueous extract (APAE) to reverse neuroinflammation and cognitive impairment induced by lipopolysaccharide (LPS) was examined in vivo. Rats were treated with APAE (50, 100, 200, and 400mg.kg(-1), p.o.) for 7 consecutive days prior to LPS (1mg.kg(-1), i.p.)-induced neuroinflammation and cognitive impairment. Spatial learning and memory were evaluated using the Morris water maze (MWM) test, while neuroinflammation and oxidative stress were assessed through the measurement of specific mediators, namely, tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-1beta, superoxide dismutase (SOD), catalase (CAT), antioxidant glutathione (GSH), reactive oxygen species (ROS), and thiobarbituric acid reactive substance (TBARS). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were also evaluated. LPS caused significant memory deficits in the 2-day MWM protocol, whereas pretreatment with standardised APAE dose-dependently improved performance in the MWM test. APAE treatment also blocked the LPS-induced hippocampal increase in the concentration and expression of proinflammatory cytokines (TNF-alpha, IL-1beta, and IL-6) and production of ROS and TBARS and enhanced the activities of AChE and BChE. Furthermore, APAE enhanced the decrease in the levels and expression of hippocampal antioxidant enzymes (SOD and CAT) following LPS-induced neuroinflammation and cognitive deficit. The findings from these studies suggested that standardised APAE improved memory and had potent neuroprotective effects against LPS-induced neurotoxicity.

PubMedSearch : Sani_2019_J.Adv.Res_16_87
PubMedID: 30899592

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Citations formats

Sani D, Khatab NIO, Kirby BP, Yong A, Hasan S, Basri H, Stanslas J (2019)
A standardised Andrographis paniculata Burm. Nees aqueous extract prevents Lipopolysaccharide-induced cognitive deficits through suppression of inflammatory cytokines and oxidative stress mediators
J Adv Res 16 :87

Sani D, Khatab NIO, Kirby BP, Yong A, Hasan S, Basri H, Stanslas J (2019)
J Adv Res 16 :87