Scarcella_2012_Mol.Biochem.Parasitol_185_151

In the present work we evaluate in vivo the activity of carboxylesterase of Fasciola hepatica exposed to triclabendazole We observed a statistically significant increase in enzyme activity at 24 and 48h post treatment P<0.01 and P<0.001 respectively The zymogram of cytosolic fractions identified a protein of 170kDa containing the carboxylesterase activity The densitograms of the zymograms confirmed the phenomenon of enzyme induction under the experimental conditions of the assay These results provide not only the understanding of the importance of this metabolic pathway in flukes but carboxylesterase would also be an enzyme that could participate more actively in the development of anthelmintic resistance at TCBZ.