Schallreuter_2006_Biochem.Biophys.Res.Commun_349_931

Reference

Title : Butyrylcholinesterase is present in the human epidermis and is regulated by H2O2: more evidence for oxidative stress in vitiligo - Schallreuter_2006_Biochem.Biophys.Res.Commun_349_931
Author(s) : Schallreuter KU , Gibbons NC , Zothner C , Elwary SM , Rokos H , Wood JM
Ref : Biochemical & Biophysical Research Communications , 349 :931 , 2006
Abstract :

The human epidermis holds the capacity for autocrine cholinergic signal transduction, but the presence of butyrylcholinesterase (BchE) has not been shown so far. Our results demonstrate that this compartment transcribes a functional BchE. Its activity is even higher compared to acetylcholinesterase (AchE). Moreover, we show that BchE is subject to regulation by H(2)O(2) in a concentration-dependent manner as it was recently described for AchE. Epidermal BchE protein expression and enzyme activities are severely affected by H(2)O(2) in vitiligo as previously demonstrated for AchE. Removal/reduction of H(2)O(2) by a pseudocatalase PC-KUS yields normal/increased protein expression and activities. H(2)O(2)-mediated oxidation of methionine residues in BchE was confirmed by FT-Raman spectroscopy. Computer simulation supported major alteration of the enzyme active site and its tetramerisation domain suggesting deactivation of the enzyme due to H(2)O(2)-mediated oxidation. Based on our results we conclude that H(2)O(2) is a major player in the regulation of the cholinergic signal via both AchE and BchE and this signal is severely affected in the epidermis of patients with active vitiligo.

PubMedSearch : Schallreuter_2006_Biochem.Biophys.Res.Commun_349_931
PubMedID: 16962996

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Citations formats

Schallreuter KU, Gibbons NC, Zothner C, Elwary SM, Rokos H, Wood JM (2006)
Butyrylcholinesterase is present in the human epidermis and is regulated by H2O2: more evidence for oxidative stress in vitiligo
Biochemical & Biophysical Research Communications 349 :931

Schallreuter KU, Gibbons NC, Zothner C, Elwary SM, Rokos H, Wood JM (2006)
Biochemical & Biophysical Research Communications 349 :931