Title : The Aspergillus nidulans enzyme TdiB catalyzes prenyltransfer to the precursor of bioactive asterriquinones - Schneider_2008_Fungal.Genet.Biol_45_302 |
Author(s) : Schneider P , Weber M , Hoffmeister D |
Ref : Fungal Genet Biol , 45 :302 , 2008 |
Abstract :
The asterriquinones represent a class of ascomycete metabolic products whose significance stems from remarkable and useful pharmacological activities, among those antiretroviral (e.g., against the HI-virus), antitumor, and antidiabetes properties. Recently, the first genetic locus for an asterriquinone, the clustered terrequinone genes tdiA-E, was identified during a genome-wide screen in Aspergillus nidulans for "orphan" natural product biosynthesis loci. Here, we describe overexpression and characterization of TdiB, which catalyzes the reverse prenylation event during terrequinone A biosynthesis, which is the transfer of dimethylallyl diphosphate to carbon atom 2' of the intermediate didemethylasterriquinone D, to yield asterriquinone C-1. TdiB does not depend on the presence of divalent metal cations for catalysis and lacks the canonical prenyl diphosphate binding motif (D/N)DXXD. |
PubMedSearch : Schneider_2008_Fungal.Genet.Biol_45_302 |
PubMedID: 18029206 |
Gene_locus related to this paper: emeni-tdia |
Gene_locus | emeni-tdia |
Schneider P, Weber M, Hoffmeister D (2008)
The Aspergillus nidulans enzyme TdiB catalyzes prenyltransfer to the precursor of bioactive asterriquinones
Fungal Genet Biol
45 :302
Schneider P, Weber M, Hoffmeister D (2008)
Fungal Genet Biol
45 :302