Schubert_2012_Life.Sci_91_1048

Reference

Title : Expression of the non-neuronal cholinergic system in human knee synovial tissue from patients with rheumatoid arthritis and osteoarthritis - Schubert_2012_Life.Sci_91_1048
Author(s) : Schubert J , Beckmann J , Hartmann S , Morhenn HG , Szalay G , Heiss C , Schnettler R , Lips KS
Ref : Life Sciences , 91 :1048 , 2012
Abstract :

AIMS: As the stimulation of the alpha7-nicotinic acetylcholine receptor (nAChR), which is present in the synovium of patients with rheumatoid arthritis (RA), leads to a decrease in pro-inflammatory cytokines, the alpha7-nAChR is being discussed as a new therapeutic target. On this background we addressed the question whether alpha7-nAChR mRNA was differentially expressed in RA compared to osteoarthritis (OA) synovial samples and whether other components of the non-neuronal cholinergic system were also present and differentially expressed in the synovium of patients with RA in comparison to OA. MAIN
METHODS: The expression of nicotinic and muscarinic acetylcholine receptors (mAChRs), choline and acetylcholine transporters, synthesising and degrading enzymes was determined in human samples of synovial tissue from patients with RA and OA using RT-PCR and immunofluorescence labelling. KEY FINDINGS: Compared to OA, patients with RA showed increased expression of nAChR subunit beta4 while a decline in subunits alpha2 and alpha4 as well as in mAChR M1R was observed. For all other nAChR subunits and mAChRs however there was no significant difference between RA and OA patients. With regard to the ACh transporters and enzymes no expressional changes were observed between OA and RA patients, except for the choline acetyltransferase (ChAT) which was only detected in OA but not in RA synovium. SIGNIFICANCE: Our results indicate that besides alpha7-nAChR other components of the non-neuronal cholinergic system are present and differentially expressed in the synovium of RA and OA patients, which makes them interesting alternative targets in the development of new strategies for RA therapy.

PubMedSearch : Schubert_2012_Life.Sci_91_1048
PubMedID: 22569293

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Citations formats

Schubert J, Beckmann J, Hartmann S, Morhenn HG, Szalay G, Heiss C, Schnettler R, Lips KS (2012)
Expression of the non-neuronal cholinergic system in human knee synovial tissue from patients with rheumatoid arthritis and osteoarthritis
Life Sciences 91 :1048

Schubert J, Beckmann J, Hartmann S, Morhenn HG, Szalay G, Heiss C, Schnettler R, Lips KS (2012)
Life Sciences 91 :1048