Scott_1984_Brain.Res_317_53

Plasma membrane fractions isolated from fetal human brain of 14-19 weeks of gestation are capable of generating a membrane potential of 30-50 mV as a response to a gradient of K+ ions. Valinomycin, a K+ conducting ionophore, does not affect the membrane potential whereas it is markedly reduced by veratridine which opens Na+ channels in excitable membranes. The membrane fractions concentrate Ca2+ by an ATP-dependent mechanism. The uptake has a high affinity for Ca2+, it is enhanced by oxalate and abolished by the 2H+/Ca2+ exchanger A 23187. Trifluoroperazine (40 microM), a calmodulin antagonist, inhibits Ca2+ uptake by 80%. Addition of Na+ causes efflux of part of the Ca2+ taken up in the presence of ATP, suggesting that a Na+-linked Ca2+ transport is also present in the membranes. The results show that the neuronal membranes of the fetal human brain already in the early second trimester of gestation have properties similar to those of the adult animal brain.