| Title : Alkynyl phosphates are potent inhibitors of serine enzyme - Segal_1996_FEBS.Lett_392_117 |
| Author(s) : Segal D , Shalitin C , Shalitin Y , Fischer DR , Stang PJ |
| Ref : FEBS Letters , 392 :117 , 1996 |
|
Abstract :
Propynyl, hexynyl and t-butylethynyl diethyl phosphates were found to be very powerful covalent inhibitors of serine enzymes. Esterases were inhibited with second-order rate constants of 10(7)-10(8) m(-1) min(-1). Most proteases were inhibited with a rate constant of 10(4)-10(5) M(-1) min(-1). By inhibiting chymotrypsin with (3-14C)-1-propynyl diethyl phosphate, it was established that inhibition was caused by binding of the phosphate group to the enzyme active site |
| PubMedSearch : Segal_1996_FEBS.Lett_392_117 |
| PubMedID: 8772187 |
Segal D, Shalitin C, Shalitin Y, Fischer DR, Stang PJ (1996)
Alkynyl phosphates are potent inhibitors of serine enzyme
FEBS Letters
392 :117
Segal D, Shalitin C, Shalitin Y, Fischer DR, Stang PJ (1996)
FEBS Letters
392 :117