Seki_2020_J.Agric.Food.Chem__

Reference

Title : Dipeptidyl peptidase-IV inhibitory activity of Katsuobushi-derived peptides in Caco-2 cell assay and oral glucose tolerance test in ICR mice - Seki_2020_J.Agric.Food.Chem__
Author(s) : Seki E , Yamamoto A , Fujiwara Y , Yamane T , Satsu H , Ohkubo I
Ref : Journal of Agricultural and Food Chemistry , : , 2020
Abstract : Proteolytic products of bonito stock residue inhibit dipeptidyl peptidase-IV (DPP-IV). Here, we isolated, purified, and identified the components of its N5 fraction obtained after using neutral protease from Aspergillus oryzae. A 10%-ethanol eluent (N5-2 fraction) from column chromatography was sequenced, yielding 18 peptides. Of these, Glu-Val-Phe, Ala-Va-Phe, and Gly-Val-Phe were identified as novel (IC50 values for DPP-IV inhibition were 525.56, 5466.49, and 2870.87 microM , respectively), whereas Trp-Val is the primary peptide (IC50 value of 36.99 microM, 1359 unit (mL/100g-N5-2 fraction) = (yield (mg/)/100g-N5-2 fraction)/ IC50(microg/mL). Furthermore, the N5-2 fraction significantly decreased DPP-IV activity in Caco-2 intestinal epithelial cells (p<0.05). From the oral glucose tolerance test using ICR mice, the N5-2 fraction significantly attenuated the rise in serum glucose levels compared with the control (p<0.05) through cell-surface DPP-IV inhibition. We discuss the novelty, the significance and relevance of finding study as well as its broad applications of prevention with diabetes.
ESTHER : Seki_2020_J.Agric.Food.Chem__
PubMedSearch : Seki_2020_J.Agric.Food.Chem__
PubMedID: 32396370

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Citations formats

Seki E, Yamamoto A, Fujiwara Y, Yamane T, Satsu H, Ohkubo I (2020)
Dipeptidyl peptidase-IV inhibitory activity of Katsuobushi-derived peptides in Caco-2 cell assay and oral glucose tolerance test in ICR mice
Journal of Agricultural and Food Chemistry :

Seki E, Yamamoto A, Fujiwara Y, Yamane T, Satsu H, Ohkubo I (2020)
Journal of Agricultural and Food Chemistry :