| Title : MGL2\/YMR210w encodes a monoacylglycerol lipase in Saccharomyces cerevisiae - Selvaraju_2016_FEBS.Lett_590_1174 |
| Author(s) : Selvaraju K , Gowsalya R , Vijayakumar R , Nachiappan V |
| Ref : FEBS Letters , 590 :1174 , 2016 |
|
Abstract :
In silico analysis of the uncharacterized open reading frame YMR210w in Saccharomyces cerevisiae revealed that it possesses both an alpha/beta hydrolase domain (ABHD) and a typical lipase (GXSXG) motif. The purified protein displayed monoacylglycerol (MAG) lipase activity and preferred palmitoyl-MAG. Overexpression of YMR210w in the known MAG lipase mutant yju3Delta clearly revealed that the protein had MAG lipase activity, hence we named the ORF MGL2. Overexpression of YMR210w decreased the cellular triacylglycerol levels. Analysis of the overexpressed strains showed reduction in the lipid droplets number and size. Phenotype studies revealed that the double deletion yju3Deltamgl2Delta displayed a growth defect that was partially restored by MGL2 overexpression. |
| PubMedSearch : Selvaraju_2016_FEBS.Lett_590_1174 |
| PubMedID: 26991558 |
| Gene_locus related to this paper: yeast-ym60 |
| Substrate | Monopalmitin |
| Gene_locus | yeast-ym60 |
Selvaraju K, Gowsalya R, Vijayakumar R, Nachiappan V (2016)
MGL2\/YMR210w encodes a monoacylglycerol lipase in Saccharomyces cerevisiae
FEBS Letters
590 :1174
Selvaraju K, Gowsalya R, Vijayakumar R, Nachiappan V (2016)
FEBS Letters
590 :1174