Title : Colorimetric Detection of Organophosphate Pesticides Based on Acetylcholinesterase and Cysteamine Capped Gold Nanoparticles as Nanozyme - Shah_2021_Sensors.(Basel)_21_ |
Author(s) : Shah MM , Ren W , Irudayaraj J , Sajini AA , Ali MI , Ahmad B |
Ref : Sensors (Basel) , 21 : , 2021 |
Abstract :
Organophosphates (OPs) are neurotoxic agents also used as pesticides that can permanently block the active site of the acetylcholinesterase (AChE). A robust and sensitive detection system of OPs utilising the enzyme mimic potential of the cysteamine capped gold nanoparticles (C-AuNPs) was developed. The detection assay was performed by stepwise addition of AChE, parathion ethyl (PE)-a candidate OP, acetylcholine chloride (ACh), C-AuNPs, and 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the buffer solution. The whole sensing protocol completes in 30-40 min, including both incubations. The Transmission Electron Microscopy (TEM) results indicated that the NPs are spherical and have an average size of 13.24 nm. The monomers of C-AuNPs exhibited intense catalytic activity (nanozyme) for the oxidization of TMB, revealed by the production of instant blue colour and confirmed by a sharp peak at 652 nm. The proposed biosensor's detection limit and linear ranges were 5.8 ng.mL(-1) and 11.6-92.8 ng.mL(-1), respectively, for PE. The results strongly advocate that the suggested facile colorimetric biosensor may provide an excellent platform for on-site monitoring of OPs. |
PubMedSearch : Shah_2021_Sensors.(Basel)_21_ |
PubMedID: 34884060 |
Shah MM, Ren W, Irudayaraj J, Sajini AA, Ali MI, Ahmad B (2021)
Colorimetric Detection of Organophosphate Pesticides Based on Acetylcholinesterase and Cysteamine Capped Gold Nanoparticles as Nanozyme
Sensors (Basel)
21 :
Shah MM, Ren W, Irudayaraj J, Sajini AA, Ali MI, Ahmad B (2021)
Sensors (Basel)
21 :