Title : Amyloid-beta induced neuropathological actions are suppressed by Padina gymnospora (Phaeophyceae) and its active constituent alpha-bisabolol in Neuro2a cells and transgenic Caenorhabditis elegans Alzheimer's model - Shanmuganathan_2019_Nitric.Oxide_91_52 |
Author(s) : Shanmuganathan B , Sathya S , Balasubramaniam B , Balamurugan K , Devi KP |
Ref : Nitric Oxide , 91 :52 , 2019 |
Abstract :
The inhibition of Abeta peptide development and aggregation is a hopeful curative approach for the discovery of disease modifying drugs for Alzheimer's disease (AD) treatment. Recent research mainly focuses on the discovery of drugs from marine setting due to their immense therapeutic potential. The present study aims to evaluate the brown macroalga Padina gymnospora and its active constituent alpha-bisabolol against Abeta25-35 induced neurotoxicity in Neuro2a cells and transgenic Caenorhabditis elegans (CL2006 and CL4176). The results of the in vitro study revealed that the acetone extract of P. gymnospora (ACTPG) and its active constituent alpha-bisabolol restores the Abeta25-35 induced alteration in the oxidation of intracellular protein and lipids. In addition, ACTPG and alpha-bisabolol inhibited cholinesterase and beta-secretase activity in Neuro2a cells. Moreover, the intracellular reactive oxygen species (ROS) and reactive nitrogen species (RNS) production was reduced by ACTPG and alpha-bisabolol in Neuro2a cells. The decrease in the expression level of apoptotic proteins such as Bax and caspase-3 in ACTPG and alpha-bisabolol treated group indicates that the seaweed and its bioactive compound have anti-apoptotic property. Further, the in vivo study revealed that the ACTPG and alpha-bisabolol exerts neuroprotective effect against Abeta induced proteotoxicity in transgenic C. elegans strains of AD. Moreover it altered the Abeta mediated pathways, lifespan, macromolecular damage and down regulated the AD related gene expression of ace-1, hsp-4 and Abeta, thereby preventing Abeta synthesis. Overall, the outcome of the study signifies the neuroprotective effect of ACTPG and alpha-bisabolol against Abeta mediated AD pathology. |
PubMedSearch : Shanmuganathan_2019_Nitric.Oxide_91_52 |
PubMedID: 31362072 |
Shanmuganathan B, Sathya S, Balasubramaniam B, Balamurugan K, Devi KP (2019)
Amyloid-beta induced neuropathological actions are suppressed by Padina gymnospora (Phaeophyceae) and its active constituent alpha-bisabolol in Neuro2a cells and transgenic Caenorhabditis elegans Alzheimer's model
Nitric Oxide
91 :52
Shanmuganathan B, Sathya S, Balasubramaniam B, Balamurugan K, Devi KP (2019)
Nitric Oxide
91 :52