Shapiro_1989_Biochemistry_28_8946

Reference

Title : Deletion analysis of the mouse m1 muscarinic acetylcholine receptor: effects on phosphoinositide metabolism and down-regulation - Shapiro_1989_Biochemistry_28_8946
Author(s) : Shapiro RA , Nathanson NM
Ref : Biochemistry , 28 :8946 , 1989
Abstract :

Deletions have been constructed in the putative third cytoplasmic loop of the mouse m1 muscarinic acetylcholine receptor (mAChR) gene, and the effects of these mutations on mAChR coupling to phosphoinositide metabolism and agonist-induced down-regulation have been examined following expression in Y1 adrenal carcinoma cells. Deletion of up to 123 of the 156 amino acids in this loop has no effect on antagonist or agonist binding, or on coupling to stimulation of phosphoinositide metabolism. These results suggest that the membrane proximal portions of this loop are involved in determining the specificity of functional coupling of the receptor. Deletion of 75% of the loop has no effect on short-term agonist-induced internalization but does cause a significant decrease in the magnitude of agonist-induced down-regulation of receptor number. Thus, this portion of the receptor may be involved in mediating the response to long-term agonist exposure.

PubMedSearch : Shapiro_1989_Biochemistry_28_8946
PubMedID: 2557912

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Citations formats

Shapiro RA, Nathanson NM (1989)
Deletion analysis of the mouse m1 muscarinic acetylcholine receptor: effects on phosphoinositide metabolism and down-regulation
Biochemistry 28 :8946

Shapiro RA, Nathanson NM (1989)
Biochemistry 28 :8946