| Title : Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor - Shapiro_1989_Proc.Natl.Acad.Sci.U.S.A_86_9039 |
| Author(s) : Shapiro RA , Wakimoto BT , Subers EM , Nathanson NM |
| Ref : Proc Natl Acad Sci U S A , 86 :9039 , 1989 |
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Abstract :
Genomic and cDNA clones encoding a muscarinic acetylcholine receptor from Drosophila melanogaster have been isolated. Sequence analysis demonstrates that this gene encodes a receptor with a high degree of amino acid identity to the mammalian muscarinic acetylcholine receptors and has three introns in the portion of the gene encoding the third putative cytoplasmic loop. A full-length cDNA clone has been placed under the control of the mouse metallothionein promotor and transfected into mouse Y1 adrenal cells. The receptor expressed in these cells exhibits the high-affinity binding for the antagonists quinuclidinyl benzilate and atropine expected of a muscarinic receptor. The Drosophila muscarinic receptor, when expressed in Y1 cells, is physiologically active, as measured by agonist-dependent stimulation of phosphatidylinositol metabolism. |
| PubMedSearch : Shapiro_1989_Proc.Natl.Acad.Sci.U.S.A_86_9039 |
| PubMedID: 2510174 |
Shapiro RA, Wakimoto BT, Subers EM, Nathanson NM (1989)
Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor
Proc Natl Acad Sci U S A
86 :9039
Shapiro RA, Wakimoto BT, Subers EM, Nathanson NM (1989)
Proc Natl Acad Sci U S A
86 :9039