| Title : Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors - Shariff_2007_J.Basic.Microbiol_47_406 |
| Author(s) : Shariff FM , Leow ATC , Mukred AD , Salleh AB , Basri M , Rahman RNZRA |
| Ref : J Basic Microbiol , 47 :406 , 2007 |
|
Abstract :
A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 degrees C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca(2+) and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 degrees C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa. |
| PubMedSearch : Shariff_2007_J.Basic.Microbiol_47_406 |
| PubMedID: 17910105 |
Shariff FM, Leow ATC, Mukred AD, Salleh AB, Basri M, Rahman RNZRA (2007)
Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors
J Basic Microbiol
47 :406
Shariff FM, Leow ATC, Mukred AD, Salleh AB, Basri M, Rahman RNZRA (2007)
J Basic Microbiol
47 :406