Sharma_2017_J.Genet.Eng.Biotechnol_15_369

Lipase production bacterial isolate was isolated from soil of service station and identified as Bacillus methylotrophicus PS3 by 16SrRNA with accession number |LN999829.1|. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatography. The molecular weight of purified enzyme was 31.40 kDa on SDS-PAGE. This purification procedure resulted in 2.90-fold purification of lipase with a 24.10% final yield. The purified lipase presented maximal hydrolytic activity at a temperature of 55 ( degrees )C, and pH of 7.0. Lipase activity was stimulated by Triton X-100 and SDS with Mg(2+) and Ca(2+) metals employ a positive effect and outlast its stable in organic solvent i.e. methanol and ethanol.