| Title : Mass spectrometry-based identification of carbohydrate anomeric configuration to determine the mechanism of glycoside hydrolases - Shen_2019_Carbohydr.Res_476_53 |
| Author(s) : Shen YH , Tsai ST , Liew CY , Ni CK |
| Ref : Carbohydr Res , 476 :53 , 2019 |
|
Abstract :
A rapid mass spectrometry method for determining the anomeric configuration of the sugar at the reducing end of an oligosaccharide was demonstrated. The method was employed to identify the nascent anomeric configuration (i.e., before significant mutarotation occurs) of oligosaccharides released by carbohydrate-active enzymes, which enabled determination of the enzyme mechanism. This method was validated by applying it to various enzymes, including alpha-glucosidase, beta-glucosidases, endoglycoceramidase II, beta-galactosidase, and beta-amylase. |
| PubMedSearch : Shen_2019_Carbohydr.Res_476_53 |
| PubMedID: 30897369 |
Shen YH, Tsai ST, Liew CY, Ni CK (2019)
Mass spectrometry-based identification of carbohydrate anomeric configuration to determine the mechanism of glycoside hydrolases
Carbohydr Res
476 :53
Shen YH, Tsai ST, Liew CY, Ni CK (2019)
Carbohydr Res
476 :53