Shi_2012_In.Vitro.Cell.Dev.Biol.Anim_48_603

Reference

Title : Lhx8 promote differentiation of hippocampal neural stem\/progenitor cells into cholinergic neurons in vitro - Shi_2012_In.Vitro.Cell.Dev.Biol.Anim_48_603
Author(s) : Shi J , Li H , Jin G , Zhu P , Tian M , Qin J , Tan X , Zhao S , Wang F , Hua Y , Xiao Y
Ref : In Vitro Cell Developmental Biology Anim , 48 :603 , 2012
Abstract :

Lhx8, also named L3, is a recently identified member of the LIM homeobox gene family. Previously, we found acetylcholinesterase (AChE)-positive cells in fimbria-fornix (FF) transected rat hippocampal subgranular zone (SGZ). In the present study, we detected choline acetyltransferase (ChAT)-positive cholinergic cells in hippocampal SGZ after FF transaction, and these ChAT-positive cells were double labeled by Lhx8. Then we overexpressed Lhx8 during neural differentiation of hippocampal neural stem/progenitor cells on adherent conditions using lentivirus Lenti6.3-Lhx8. The result indicated that overexpression of Lhx8 did not affect the proportion of MAP2-positive neurons, but increased the proportion of ChAT-positive cells in vitro. These results suggested that FF-transected hippocampal niche promoted the ChAT/Lhx8-positive cholinergic neurons generation in rodent hippocampus, and Lhx8 was not associated with the MAP2-positive neurons differentiation on adherent conditions, but played a role in the specification of cholinergic neurons derived from hippocampal neural stem/progenitor cells in vitro.

PubMedSearch : Shi_2012_In.Vitro.Cell.Dev.Biol.Anim_48_603
PubMedID: 23150137

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Citations formats

Shi J, Li H, Jin G, Zhu P, Tian M, Qin J, Tan X, Zhao S, Wang F, Hua Y, Xiao Y (2012)
Lhx8 promote differentiation of hippocampal neural stem\/progenitor cells into cholinergic neurons in vitro
In Vitro Cell Developmental Biology Anim 48 :603

Shi J, Li H, Jin G, Zhu P, Tian M, Qin J, Tan X, Zhao S, Wang F, Hua Y, Xiao Y (2012)
In Vitro Cell Developmental Biology Anim 48 :603