| Title : Insect-vertebrate chimeric nicotinic acetylcholine receptors identify a region, loop B to the N-terminus of the Drosophila Dalpha2 subunit, which contributes to neonicotinoid sensitivity - Shimomura_2005_Neurosci.Lett_385_168 |
| Author(s) : Shimomura M , Satoh H , Yokota M , Ihara M , Matsuda K , Sattelle DB |
| Ref : Neuroscience Letters , 385 :168 , 2005 |
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Abstract :
A chimera based on the chicken alpha4 nicotinic acetylcholine receptor (nAChR) subunit containing an insert from loop B to the N-terminus of the Drosophila melanogaster Dalpha2 (=SAD) subunit was constructed and co-expressed with the chicken beta2 nAChR subunit in Xenopus laevis oocytes. The actions of the neonicotinoid insecticide imidacloprid were examined. Replacement of the region loop B to the N-terminus of the alpha4 subunit by the corresponding region of the Dalpha2 subunit had little effect on the concentration-response curve for imidacloprid. However, replacement of Glu219 by proline in the YXCC motif in loop C of the chimeric alpha4 subunit resulted in a marked displacement to the left of the concentration-response curve for imidacloprid not seen when an equivalent mutation was made in the alpha4beta2 nAChR. The results suggest that the region loop B to the N-terminus in the Dalpha2 subunit contributes to the high imidacloprid sensitivity of the hybrid Dalpha2beta2 nAChR. |
| PubMedSearch : Shimomura_2005_Neurosci.Lett_385_168 |
| PubMedID: 15963641 |
Shimomura M, Satoh H, Yokota M, Ihara M, Matsuda K, Sattelle DB (2005)
Insect-vertebrate chimeric nicotinic acetylcholine receptors identify a region, loop B to the N-terminus of the Drosophila Dalpha2 subunit, which contributes to neonicotinoid sensitivity
Neuroscience Letters
385 :168
Shimomura M, Satoh H, Yokota M, Ihara M, Matsuda K, Sattelle DB (2005)
Neuroscience Letters
385 :168