Shin_1998_J.Chromatogr.B.Biomed.Sci.Appl_718_61

Reference

Title : Simultaneous determination of flupyrazofos and its metabolite 1-phenyl-3-trifluoromethyl-5-hydroxypyrazole and flupyrazofos oxon in rat plasma by high-performance liquid chromatography with ultraviolet absorbance detection - Shin_1998_J.Chromatogr.B.Biomed.Sci.Appl_718_61
Author(s) : Shin HC , Shim HO , Lee YM , Song SW , Kim JH , Chung MK , Han SS , Roh JK
Ref : Journal of Chromatography B Biomed Sci Appl , 718 :61 , 1998
Abstract :

An isocratic high-performance liquid chromatography (HPLC) system with UV detection was developed for simultaneous determination of flupyrazofos and its metabolites, 1-phenyl-3-trifluoromethyl-5-hydroxypyrazole and flupyrazofos oxon, in rat plasma. Optimal analytical conditions involved an analytical cartridge column consisting of a phenyl bonded phase, a mobile phase of 50 mM phosphate buffer (pH3.0)-acetonitrile (40:60, v/v) and a UV detection wavelength of 232 nm. Under these conditions the peaks of flupyrazofos and its metabolites were all well separated and the total time for complete separation was less than 12 min. The limit of quantitation was 40 ng/ml for flupyrazofos and 20 ng/ml for PTMHP. Recoveries from rat plasma were higher than 90%. Following intravenous administration of flupyrazofos, the method has been successfully applied in a toxicokinetic study in rats involving plasma samples. Therefore, the current method is a valuable analytical tool for investigating the metabolism and toxicokinetics of flupyrazofos.

PubMedSearch : Shin_1998_J.Chromatogr.B.Biomed.Sci.Appl_718_61
PubMedID: 9832361

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Citations formats

Shin HC, Shim HO, Lee YM, Song SW, Kim JH, Chung MK, Han SS, Roh JK (1998)
Simultaneous determination of flupyrazofos and its metabolite 1-phenyl-3-trifluoromethyl-5-hydroxypyrazole and flupyrazofos oxon in rat plasma by high-performance liquid chromatography with ultraviolet absorbance detection
Journal of Chromatography B Biomed Sci Appl 718 :61

Shin HC, Shim HO, Lee YM, Song SW, Kim JH, Chung MK, Han SS, Roh JK (1998)
Journal of Chromatography B Biomed Sci Appl 718 :61