Title : Biological insights and NMR metabolic profiling of different extracts of Spermacoce verticillata (L.) G. Mey - Sinan_2021_Chem.Biodivers__ |
Author(s) : Sinan KI , Zengin G , Fiorentino A , D'Abrosca B , Ak G , Lobine D , Etienne OK , Subratty AH , Mahomoodally F |
Ref : Chem Biodivers , : , 2021 |
Abstract :
Spermacoce verticillata (L.) G. Mey. is commonly used in the folk medicine by various cultures to manage common diseases. Herein, the chemical and biological profiles of S. verticillata were studied in order to provide a comprehensive characterization of bioactive compounds and also to highlight the therapeutic properties. The in vitro antioxidant activity using free-radical scavenging, phosphomolybdenum, ferrous-ion chelating and reducing power assays, and the inhibitory activity against key enzymes such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), tyrosinase, alpha-amylase and alpha-glucosidase of S. verticillata extracts (dichloromethane, ethyl acetate, methanol and water) were investigated. The highest total phenolic and flavonoid content were observed in the methanolic and aqueous extracts. Exhaustive 2DNMR investigation has revealed the presence of rutin, ursolic and oleanoic acids. The methanolic extract, followed by aqueous extract have showed remarkable free radical quenching and reducing ability, while the dichloromethane extract was the best source of metal chelators. The tested extracts showed notable inhibitory activity against cholinesterases (AChE: 1.63 - 4.99 mg GALAE/g extract and BChE: 12.40 - 15.48 mg GALAE/g extract) and tyrosinase (60.85 - 159.64 mg KAE/g extract). No inhibitory activity was displayed by ethyl acetate and aqueous extracts against BChE and tyrosinase, respectively. All the tested extracts showed modest alpha-amylase inhibitory activity, while only the ethyl acetate and aqueous extracts were potent against alpha-glycosidase. This study further validates the use of S. verticillata in the traditional medicine, while advocating for further investigation for phytomedicine development. |
PubMedSearch : Sinan_2021_Chem.Biodivers__ |
PubMedID: 34390173 |
Sinan KI, Zengin G, Fiorentino A, D'Abrosca B, Ak G, Lobine D, Etienne OK, Subratty AH, Mahomoodally F (2021)
Biological insights and NMR metabolic profiling of different extracts of Spermacoce verticillata (L.) G. Mey
Chem Biodivers
:
Sinan KI, Zengin G, Fiorentino A, D'Abrosca B, Ak G, Lobine D, Etienne OK, Subratty AH, Mahomoodally F (2021)
Chem Biodivers
: