Singh_2024_J.Biochem.Mol.Toxicol_38_e70046

Caloric restriction mimetics mimic the beneficial effects of dietary restriction approaches, such as caloric restriction approaches, without limiting the dietary intake. Chlorogenic acid (CGA) acts as a caloric restriction mimetic; however, its neuroprotective mechanisms remain ambiguous. Therefore, in this study, we aimed to elucidate the neuroprotective effects of CGA on SH-SY5Y cells treated with amyloid-beta (Abeta)1-42. Abeta1-42 (1.25 microM) induced oxidative stress by significantly increasing the pro-oxidant levels and decreasing the antioxidant levels in the cells. Additionally, it decreased the Na(+)/K(+)-ATPase and Ca(2+)-ATPase ion transporter activities and enhanced the acetylcholinesterase activity. However, CGA (100 microM) reversed these Abeta1-42-induced changes in SH-SY5Y cells. Overall, CGA exerted neuroprotective effects against Abeta1-42-induced oxidative stress and impaired ion transporter and acetylcholinesterase activities, serving as a promising therapeutic candidate for neurodegenerative diseases, such as Alzheimer's disease.