| Title : Studies on immobilized lipase in hydrophobic sol-gel - Soares_2004_Appl.Biochem.Biotechnol_113-116_307 |
| Author(s) : Soares CMF , dos Santos OA , de Castro HF , de Moraes FF , Zanin GM |
| Ref : Appl Biochem Biotechnol , 113-116 :307 , 2004 |
|
Abstract :
The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for the immobilization of lipase from Candida rugosa by three methods: physical adsorption, covalent binding, and gel entrapment in the presence and absence of polyethylene glycol (PEG-1450). The silica matrices and their derivatives were characterized regarding particle size distribution, specific surface area, pore size distribution (Brunauer, Emmett, and Teller [B.E.T.] method), yield of grafting (thermogravimetric analyzer [TGA]), and chemical composition (Fourier transform infrared). Immobilization yields based on recovered lipase activity varied from 3.0 to 32.0%, and the highest efficiency was attained when lipase was encapsulated in the presence of PEG. |
| PubMedSearch : Soares_2004_Appl.Biochem.Biotechnol_113-116_307 |
| PubMedID: 15054215 |
Soares CMF, dos Santos OA, de Castro HF, de Moraes FF, Zanin GM (2004)
Studies on immobilized lipase in hydrophobic sol-gel
Appl Biochem Biotechnol
113-116 :307
Soares CMF, dos Santos OA, de Castro HF, de Moraes FF, Zanin GM (2004)
Appl Biochem Biotechnol
113-116 :307