| Title : Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216 - Song_2006_Protein.Expr.Purif_47_393 |
| Author(s) : Song HT , Jiang ZB , Ma LX |
| Ref : Protein Expr Purif , 47 :393 , 2006 |
|
Abstract :
We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized. |
| PubMedSearch : Song_2006_Protein.Expr.Purif_47_393 |
| PubMedID: 16624574 |
| Gene_locus related to this paper: yarli-LIP7 , yarli-LIP8 |
| Gene_locus | yarli-LIP7 yarli-LIP8 |
Song HT, Jiang ZB, Ma LX (2006)
Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216
Protein Expr Purif
47 :393
Song HT, Jiang ZB, Ma LX (2006)
Protein Expr Purif
47 :393