Title : Distinct mechanisms for the endocytosis of muscarinic receptors and Gq\/11 - Sorensen_1999_Eur.J.Pharmacol_372_325 |
Author(s) : Sorensen SD , Linseman DA , Fisher SK |
Ref : European Journal of Pharmacology , 372 :325 , 1999 |
Abstract :
To determine whether the agonist-mediated endocytosis of muscarinic receptors and Gq/11 are mechanistically related events, the internalization of Gq/11 was monitored under conditions established to prevent muscarinic receptor endocytosis. Incubation of SH-SY5Y neuroblastoma cells with oxotremorine-M resulted in the translocation of both muscarinic receptors and Gq/11 into a 'light vesicle' membrane fraction. Although muscarinic receptor translocation was prevented by either the depletion of phosphoinositides or by disruption of clathrin assembly, the endocytosis of Gq/11 was unaffected. These results demonstrate that the agonist-induced internalization of muscarinic receptors and Gq/11 proceed via distinct mechanisms. |
PubMedSearch : Sorensen_1999_Eur.J.Pharmacol_372_325 |
PubMedID: 10395032 |
Sorensen SD, Linseman DA, Fisher SK (1999)
Distinct mechanisms for the endocytosis of muscarinic receptors and Gq\/11
European Journal of Pharmacology
372 :325
Sorensen SD, Linseman DA, Fisher SK (1999)
European Journal of Pharmacology
372 :325