| Title : Optimizing bioconversion pathways through systems analysis and metabolic engineering - Stafford_2002_Proc.Natl.Acad.Sci.U.S.A_99_1801 |
| Author(s) : Stafford DE , Yanagimachi KS , Lessard PA , Rijhwani SK , Sinskey AJ , Stephanopoulos G |
| Ref : Proc Natl Acad Sci U S A , 99 :1801 , 2002 |
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Abstract :
We demonstrate a general approach for metabolic engineering of biocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic tracers for the quantification of pathway fluxes. Flux determination allows the identification of target pathways for modification as validated by subsequent overexpression of the corresponding gene. We demonstrate this method in the indene bioconversion network of Rhodococcus modified for the overproduction of 1,2-indandiol, a key precursor for the AIDS drug Crixivan. |
| PubMedSearch : Stafford_2002_Proc.Natl.Acad.Sci.U.S.A_99_1801 |
| PubMedID: 11854482 |
Stafford DE, Yanagimachi KS, Lessard PA, Rijhwani SK, Sinskey AJ, Stephanopoulos G (2002)
Optimizing bioconversion pathways through systems analysis and metabolic engineering
Proc Natl Acad Sci U S A
99 :1801
Stafford DE, Yanagimachi KS, Lessard PA, Rijhwani SK, Sinskey AJ, Stephanopoulos G (2002)
Proc Natl Acad Sci U S A
99 :1801