Stevensen_2025_ACS.Omega_10_6385

Polyethylene terephthalate (PET), a widely used plastic, is a significant environmental pollutant due to its persistence. While the PET-degrading enzyme PETase from Ideonella sakaiensis offers promising solutions, its limited activity at higher temperatures hinders its practical application. This study aimed to enhance the PETase performance through protein engineering. We introduced multiple amino acid substitutions to the wild-type I. sakaiensis PETase to improve its thermostability, substrate binding, and catalytic activity. Several potential mutant IsPETases were generated using computational design and evaluated in silico. The selected mutant was then produced in E. coli BL21(DE3). Finally, the catalytic activity of the purified mutant IsPETase was examined in vitro using p-nitrophenyl butyrate and PET substrates. IsPETase(MT) has been confirmed to be catalytically active and more thermostable with a maximum temperature reaching 60 degreesC and the T (m) value increasing up to 15.3 degreesC compared to the wild-type PETase, IsPETase(WT). IsPETase(MT) also showed better degradation toward the PET plastic film in comparison to IsPETase(WT). Thus, these findings demonstrate successful protein engineering to create a more robust PETase for potential plastic waste management applications.