Title : Nanoporous gold for enzyme immobilization - Stine_2011_Methods.Mol.Biol_679_67 |
Author(s) : Stine KJ , Jefferson K , Shulga OV |
Ref : Methods Mol Biol , 679 :67 , 2011 |
Abstract : Nanoporous gold (NPG) is a material of emerging interest for immobilization of biomolecules and -especially enzymes. NPG materials provide a high gold surface area onto which biomolecules can either be directly physisorbed or covalently linked after first modifying the NPG with a self-assembled monolayer. The material can be used as a high surface area electrode and with immobilized enzymes can be used for amperometric detection schemes. NPG can be prepared in a variety of formats from alloys containing less than 50 atomic% gold by dealloying procedures. Related high surface area gold structures have been prepared using templating approaches. Covalent enzyme immobilization can be achieved by first forming a self-assembled monolayer on NPG bearing a terminal reactive functional group followed by conjugation to the enzyme through amide linkages to lysine residues. |
ESTHER : Stine_2011_Methods.Mol.Biol_679_67 |
PubMedSearch : Stine_2011_Methods.Mol.Biol_679_67 |
PubMedID: 20865389 |
Stine KJ, Jefferson K, Shulga OV (2011)
Nanoporous gold for enzyme immobilization
Methods Mol Biol
679 :67
Stine KJ, Jefferson K, Shulga OV (2011)
Methods Mol Biol
679 :67
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