| Title : Cloning and characterization of the lipase and lipase activator protein from Vibrio vulnificus CKM-1 - Su_2004_Biochim.Biophys.Acta_1678_7 |
| Author(s) : Su JH , Chang MC , Lee YS , Tseng IC , Chuang YC |
| Ref : Biochimica & Biophysica Acta , 1678 :7 , 2004 |
| Abstract : The gene (lipA) encoding the extracellular lipase and its downstream gene (lipB) from Vibrio vulnificus CKM-1 were cloned and sequenced. Nucleotide sequence analysis and alignments of amino acid sequences suggest that Lip Ais a member of bacterial lipase family I.1 and that LipB is a lipase activator of LipA. The active LipA was produced in recombinant Escherichia coli cells only in the presence of the lipB. In the hydrolysis of p-nitrophenyl esters and triacylglycerols, using the reactivated LipA, the optimum chain lengths for the acyl moiety on the substrate were C14 for ester hydrolysis and C10 to C12 for triacylglycerol hydrolysis. |
| ESTHER : Su_2004_Biochim.Biophys.Acta_1678_7 |
| PubMedSearch : Su_2004_Biochim.Biophys.Acta_1678_7 |
| PubMedID: 15093133 |
| Gene_locus related to this paper: vibvu-VV12349 |
| Gene_locus related to this paper: vibvu-VV12349 |
Su JH, Chang MC, Lee YS, Tseng IC, Chuang YC (2004)
Cloning and characterization of the lipase and lipase activator protein from Vibrio vulnificus CKM-1
Biochimica & Biophysica Acta
1678 :7
Su JH, Chang MC, Lee YS, Tseng IC, Chuang YC (2004)
Biochimica & Biophysica Acta
1678 :7