Title : Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds - Suenaga_2007_Environ.Microbiol_9_2289 |
Author(s) : Suenaga H , Ohnuki T , Miyazaki K |
Ref : Environ Microbiol , 9 :2289 , 2007 |
Abstract :
A metagenomic approach was taken to retrieve catabolic operons for aromatic compounds from activated sludge used to treat coke plant wastewater. Metagenomic DNA extracted from the sludge was cloned into fosmids and the resulting Escherichia coli library was screened for extradiol dioxygenases (EDOs) using catechol as a substrate, yielding 91 EDO-positive clones. Based on their substrate specificity for various catecholic compounds, 38 clones were subjected to sequence analysis. Each insert contained at least one EDO gene, and a total of 43 EDO genes were identified. More than half of these belonged to new EDO subfamilies: I.1.C (2 clones), I.2.G (20 clones), I.3.M (2 clones) and I.3.N (1 clone). The fact that novel I.2.G family genes were over-represented in these clones suggested that these genes play a specific role in environmental aromatic degradation. The I.2.G clones were further classified into six groups based on single-nucleotide polymorphisms (SNPs). Based on the combination of the SNPs, the evolutionary lineage of the genes was reconstructed; further, taking the activities of the clones into account, potential adaptive mutations were identified. The metagenomic approach was thus used to retrieve novel EDO genes as well as to gain insights into the gene evolution of EDOs. |
PubMedSearch : Suenaga_2007_Environ.Microbiol_9_2289 |
PubMedID: 17686025 |
Gene_locus related to this paper: 9bact-c6kti0 , 9bact-c6ktz1 , 9bact-c6ku06 , 9bact-c6ku85 , 9bact-c6kub4 , 9bact-c6kud7 , 9bact-c6kwi7 , 9bact-c6l0y5 , 9burk-q0gqt7 , psepu-TDNF |
Gene_locus | 9bact-c6kti0 9bact-c6ktz1 9bact-c6ku06 9bact-c6ku85 9bact-c6kub4 9bact-c6kud7 9bact-c6kwi7 9bact-c6l0y5 9burk-q0gqt7 psepu-TDNF |
Gene_locus_frgt | 9bact-c6kwi3 9bact-c6l1j3 |
Suenaga H, Ohnuki T, Miyazaki K (2007)
Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds
Environ Microbiol
9 :2289
Suenaga H, Ohnuki T, Miyazaki K (2007)
Environ Microbiol
9 :2289