Sulong_2006_Protein.Expr.Purif_49_190

Reference

Title : A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene - Sulong_2006_Protein.Expr.Purif_49_190
Author(s) : Sulong MR , Rahman RNZRA , Salleh AB , Basri M
Ref : Protein Expr Purif , 49 :190 , 2006
Abstract :

UNLABELLED: An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205y lipase revealed homogeneity on denaturing gel electrophoresis and the molecular mass was at approximately 30 kDa. The optimum pH for the purified 205y lipase was 7.0-8.0 and its stability showed a broad range of pH value between pH 5.0 to 13.0 at 37 degrees C. The purified 205y lipase exhibited an optimum temperature of 55 degrees C. The activity of the purified lipase was stimulated in the presence of Ca2+ and Mg2+. Ethylenediaminetetraacetic acid (EDTA) has no effect on its activity; however inhibition was observed with phenylmethane sulfonoyl fluoride (PMSF) a serine hydrolase inhibitor. Organic solvents such as dimethylsulfoxide (DMSO), methanol, p-xylene and n-decane enhanced the activity. Studies on the effect of oil showed that the lipase was most active in the presence of tricaprin (C10). The lipase exhibited 1,3 positional specificity. KEYWORDS: Bacter

PubMedSearch : Sulong_2006_Protein.Expr.Purif_49_190
PubMedID: 16769222
Gene_locus related to this paper: bacsh-Q8VQP2

Related information

Substrate Tricaprin
Gene_locus Tricaprin    bacsh-Q8VQP2

Citations formats

Sulong MR, Rahman RNZRA, Salleh AB, Basri M (2006)
A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene
Protein Expr Purif 49 :190

Sulong MR, Rahman RNZRA, Salleh AB, Basri M (2006)
Protein Expr Purif 49 :190