Title : A Thermostable Monoacylglycerol Lipase from Marine Geobacillus sp. 12AMOR1: Biochemical Characterization and Mutagenesis Study - Tang_2019_Int.J.Mol.Sci_20_ |
Author(s) : Tang W , Lan D , Zhao Z , Li S , Li X , Wang Y |
Ref : Int J Mol Sci , 20 : , 2019 |
Abstract :
Lipases with unique substrate specificity are highly desired in biotechnological applications. In this study, a putative marine Geobacillus sp. monoacylglycerol lipase (GMGL) encoded gene was identified by a genomic mining strategy. The gene was expressed in Escherichia coli as a His-tag fusion protein and purified by affinity chromatography with a yield of 264 mg per liter fermentation broth. The recombinant GMGL shows the highest hydrolysis activity at 60 degrees C and pH 8.0, and the half-life was 60 min at 70 degrees C. The GMGL is active on monoacylglycerol (MAG) substrate but not diacylglycerol (DAG) or triacylglycerol (TAG), and produces MAG as the single product in the esterification reaction. Modeling structure analysis showed that the catalytic triad is formed by Ser97, Asp196 and His226, and the flexible cap region is constituted by residues from Ala120 to Thr160. A mutagenesis study on Leu142, Ile145 and Ile170 located in the substrate binding tunnel revealed that these residues were related with its substrate specificity. The kcat/Km value toward the pNP-C6 substrate in mutants Leu142Ala, Ile145Ala and Ile170Phe increased to 2.3-, 1.4- and 2.2-fold as compared to that of the wild type, respectively. |
PubMedSearch : Tang_2019_Int.J.Mol.Sci_20_ |
PubMedID: 30759774 |
Gene_locus related to this paper: 9baci-a0a0g3xxb4 |
Gene_locus | 9baci-a0a0g3xxb4 |
Tang W, Lan D, Zhao Z, Li S, Li X, Wang Y (2019)
A Thermostable Monoacylglycerol Lipase from Marine Geobacillus sp. 12AMOR1: Biochemical Characterization and Mutagenesis Study
Int J Mol Sci
20 :
Tang W, Lan D, Zhao Z, Li S, Li X, Wang Y (2019)
Int J Mol Sci
20 :