| Title : Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase\/Lipase Activity - Tapizquent_2017_Methods.Mol.Biol_1626_295 |
| Author(s) : Tapizquent M , Fernandez M , Barreto G , Hernandez Z , Contreras LM , Kurz L , Wilkesman J |
| Ref : Methods Mol Biol , 1626 :295 , 2017 |
|
Abstract :
Lipases are esterases that occur widely in nature, yet those with commercial relevance are exclusively from microbial origin. Glycerol and long-chain fatty acids are the products after hydrolysis of esters bonds in saponifiable lipids catalyzed by lipases. In this work, we describe lipase/esterase activity contained in cell-free fractions from thermophilic bacteria, cultured in medium containing olive oil. Analysis of the cell-free fractions by electrotransference zymography, using tributyrin as substrate, revealed bands corresponding to lipase activity. The method is simple, fast, and inexpensive. |
| PubMedSearch : Tapizquent_2017_Methods.Mol.Biol_1626_295 |
| PubMedID: 28608222 |
Tapizquent M, Fernandez M, Barreto G, Hernandez Z, Contreras LM, Kurz L, Wilkesman J (2017)
Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase\/Lipase Activity
Methods Mol Biol
1626 :295
Tapizquent M, Fernandez M, Barreto G, Hernandez Z, Contreras LM, Kurz L, Wilkesman J (2017)
Methods Mol Biol
1626 :295