Tayebati_2003_Neurosci_122_205

Reference

Title : Localization of the m5 muscarinic cholinergic receptor in rat circle of Willis and pial arteries - Tayebati_2003_Neurosci_122_205
Author(s) : Tayebati SK , Di Tullio MA , Tomassoni D , Amenta F
Ref : Neuroscience , 122 :205 , 2003
Abstract :

The expression and microanatomical localization of the muscarinic cholinergic m5 receptor subtype was investigated in rat circle of Willis and pial arteries by in situ hybridization, immunoblotting and immunohistochemistry. In situ hybridization histochemistry revealed a strong signal in the endothelium of circle of Willis and pial arteries and a moderate signal in the tunica media of the same arteries, within smooth muscle. Exposure of membranes of arteries to anti-m5 receptor protein antibodies caused the development of a band of approximately 81 kDa. Immunohistochemistry revealed the accumulation of m5 receptor protein immunoreactivity primarily within endothelium of circle of Willis and cerebral arteries and to a lesser extent in the tunica media, within smooth muscle. Medium (external diameter 200-100 microm) and small-sized (external diameter smaller than 100 microm) pial arteries displayed a significantly higher immune staining than large-sized pial arteries or circle of Willis arteries. The above data that are consistent with recent functional studies reporting cholinergic dilation of cerebral blood vessels mediated via a m5 receptor, have shown that both endothelial and muscular components of cerebral arteries synthesize and express a muscarinic m5 receptor. In view of the peculiar localization in cerebral vessels, handling of the muscarinic m5 receptor may be considered as an approach in the treatment of cerebrovascular disease.

PubMedSearch : Tayebati_2003_Neurosci_122_205
PubMedID: 14596861

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Citations formats

Tayebati SK, Di Tullio MA, Tomassoni D, Amenta F (2003)
Localization of the m5 muscarinic cholinergic receptor in rat circle of Willis and pial arteries
Neuroscience 122 :205

Tayebati SK, Di Tullio MA, Tomassoni D, Amenta F (2003)
Neuroscience 122 :205