| Title : Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S - Tayyab_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_355 |
| Author(s) : Tayyab M , Rashid N , Angkawidjaja C , Kanaya S , Akhtar M |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 69 :355 , 2013 |
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Abstract :
A thermoalkalophilic lipase (LIPSBS) from the newly isolated Geobacillus strain SBS-4S which hydrolyzes a wide range of fatty acids has been characterized. In the present study, the crystallization of purified LIPSBS using the sitting-drop vapour-diffusion method and its X-ray diffraction studies are described. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 55.13, b = 71.75, c = 126.26A. The structure was determined at 1.6A resolution by the molecular-replacement method using the lipase from G. stearothermophilus L1 as a model. |
| PubMedSearch : Tayyab_2013_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_69_355 |
| PubMedID: 23545637 |
| Gene_locus related to this paper: bacsp-lip |
Tayyab M, Rashid N, Angkawidjaja C, Kanaya S, Akhtar M (2013)
Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S
Acta Crystallographica Sect F Struct Biol Cryst Commun
69 :355
Tayyab M, Rashid N, Angkawidjaja C, Kanaya S, Akhtar M (2013)
Acta Crystallographica Sect F Struct Biol Cryst Commun
69 :355