Temeyer_2023_Int.J.Mol.Sci_24_7681

Reference

Title : Identification, Baculoviral Expression, and Biochemical Characterization of a Novel Cholinesterase of Amblyomma americanum (Acari: Ixodidae) - Temeyer_2023_Int.J.Mol.Sci_24_7681
Author(s) : Temeyer KB , Schlechte KG , Gross AD , Lohmeyer KH
Ref : Int J Mol Sci , 24 :7681 , 2023
Abstract :

A cDNA encoding a novel cholinesterase (ChE, EC 3.1.1.8) from the larvae of Amblyomma americanum (Linnaeus) was identified, sequenced, and expressed in Sf21 insect cell culture using the baculoviral expression vector pBlueBac4.5/V5-His. The open reading frame (1746 nucleotides) of the cDNA encoded 581 amino acids beginning with the initiation codon. Identical cDNA sequences were amplified from the total RNA of adult tick synganglion and salivary gland, strongly suggesting expression in both tick synganglion and saliva. The recombinant enzyme (rAaChE1) was highly sensitive to eserine and BW284c51, relatively insensitive to tetraisopropyl pyrophosphoramide (iso-OMPA) and ethopropazine, and hydrolyzed butyrylthiocholine (BuTCh) 5.7 times as fast as acetylthiocholine (ATCh) at 120 microM, with calculated KM values for acetylthiocholine (ATCh) and butyrylthiocholine of 6.39 microM and 14.18 microM, respectively. The recombinant enzyme was highly sensitive to inhibition by malaoxon, paraoxon, and coroxon in either substrate. Western blots using polyclonal rabbit antibody produced by immunization with a peptide specific for rAaChE1 exhibited reactivity in salivary and synganglial extract blots, indicating the presence of AaChE1 antigenic protein. Total cholinesterase activities of synganglial or salivary gland extracts from adult ticks exhibited biochemical properties very different from the expressed rAaACh1 enzyme, evidencing the substantial presence of additional cholinesterase activities in tick synganglion and saliva. The biological function of AaChE1 remains to be elucidated, but its presence in tick saliva is suggestive of functions in hydrolysis of cholinergic substrates present in the large blood mean and potential involvement in the modulation of host immune responses to tick feeding and introduced pathogens.

PubMedSearch : Temeyer_2023_Int.J.Mol.Sci_24_7681
PubMedID: 37175388
Gene_locus related to this paper: ambam-AaChE1

Related information

Gene_locus ambam-AaChE1

Citations formats

Temeyer KB, Schlechte KG, Gross AD, Lohmeyer KH (2023)
Identification, Baculoviral Expression, and Biochemical Characterization of a Novel Cholinesterase of Amblyomma americanum (Acari: Ixodidae)
Int J Mol Sci 24 :7681

Temeyer KB, Schlechte KG, Gross AD, Lohmeyer KH (2023)
Int J Mol Sci 24 :7681