Terholsen_2023_ChemSusChem_16_e202300168

Reference

Title : Chemoenzymatic Cascade Reaction for the Valorization of the Lignin Depolymerization Product G-C2-Dioxolane Phenol - Terholsen_2023_ChemSusChem_16_e202300168
Author(s) : Terholsen H , Meyer JRH , Zhang Z , Deuss PJ , Bornscheuer UT
Ref : ChemSusChem , 16 :e202300168 , 2023
Abstract :

Combining solid acid catalysts with enzyme reactions in aqueous environments is challenging because either very acidic conditions inactivate the enzymes, or the solid acid catalyst is neutralized. In this study, Amberlyst-15 encapsulated in polydimethylsiloxane (Amb-15@PDMS) is used to deprotect the lignin depolymerization product G-C2 dioxolane phenol in a buffered system at pH 6.0. This reaction is directly coupled with the biocatalytic reduction of the released homovanillin to homovanillyl alcohol by recombinant horse liver alcohol dehydrogenase, which is subsequently acylated by the promiscuous acyltransferase/hydrolase PestE_I208A_L209F_N288A in a one-pot system. The deprotection catalyzed with Amb-15@PDMS attains up to 97 % conversion. Overall, this cascade enables conversions of up to 57 %.

PubMedSearch : Terholsen_2023_ChemSusChem_16_e202300168
PubMedID: 36826410
Gene_locus related to this paper: pyrca-PCEST

Related information

Gene_locus pyrca-PCEST

Citations formats

Terholsen H, Meyer JRH, Zhang Z, Deuss PJ, Bornscheuer UT (2023)
Chemoenzymatic Cascade Reaction for the Valorization of the Lignin Depolymerization Product G-C2-Dioxolane Phenol
ChemSusChem 16 :e202300168

Terholsen H, Meyer JRH, Zhang Z, Deuss PJ, Bornscheuer UT (2023)
ChemSusChem 16 :e202300168