| Title : Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger - Trimukhe_2008_Int.J.Biol.Macromol_43_422 |
| Author(s) : Trimukhe KD , Mahadik ND , Gokhale DV , Varma AJ |
| Ref : Int J Biol Macromol , 43 :422 , 2008 |
|
Abstract :
Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications. |
| PubMedSearch : Trimukhe_2008_Int.J.Biol.Macromol_43_422 |
| PubMedID: 18789352 |
Trimukhe KD, Mahadik ND, Gokhale DV, Varma AJ (2008)
Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger
Int J Biol Macromol
43 :422
Trimukhe KD, Mahadik ND, Gokhale DV, Varma AJ (2008)
Int J Biol Macromol
43 :422