Tsagkarakou_2010_Pestic.Biochem.Physiol_94_49

Reference

Title : Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) - Tsagkarakou_2010_Pestic.Biochem.Physiol_94_49
Author(s) : Tsagkarakou A , Nikou Dimitra , Roditakis E , Sharvit M , Morin S , Vontas J
Ref : Pesticide Biochemistry and Physiology , 94 :49 , 2009
Abstract :

Pyrethroid and organophosphate resistance-associated mutations have been recently reported in the whitefly Bemisia tabaci (Gennadius), a major pest of protected and outdoor crops worldwide. Here, we developed simple PCR-agarose gel visualization based assays for reliably monitoring the L925I and T929V pyrethroid resistance mutations in the B. tabaci para-type voltage gated sodium channel and the iAChE F331W organophosphate resistance mutation in the acetylcholinesterase enzyme ace1. PCR-RFLP assays were developed for detecting the L925I and the F331W resistance mutations. A highly specific PASA was developed for detecting the T929V mutation. The molecular diagnostic tools were used to monitor the frequency of the resistance mutations in a large number of field caught Q biotype B. tabaci from Crete (Greece), where both organophosphates and pyrethroids are extensively used. The F331W mutation was fixed in all field individuals examined. The pyrethroid resistance mutations were detected in high frequencies: 0.38 and 0.54 for L925I and T929V, respectively. The simple diagnostics are accurate and robust, to be used alongside classical bioassays to prevent ineffective insecticide applications, and for early identification of the spreading of resistant Q biotype populations into new regions around the globe.

PubMedSearch : Tsagkarakou_2010_Pestic.Biochem.Physiol_94_49
PubMedID:
Gene_locus related to this paper: bemta-ACHE1

Related information

Mutation F392W_bemta-ACHE1
Gene_locus F392W_bemta-ACHE1    bemta-ACHE1

Citations formats

Tsagkarakou A, Nikou Dimitra, Roditakis E, Sharvit M, Morin S, Vontas J (2009)
Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)
Pesticide Biochemistry and Physiology 94 :49

Tsagkarakou A, Nikou Dimitra, Roditakis E, Sharvit M, Morin S, Vontas J (2009)
Pesticide Biochemistry and Physiology 94 :49