| Title : Biosynthesis and compositional regulation of poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in recombinant ralstonia eutropha expressing mutated polyhydroxyalkanoate synthase genes - Tsuge_2004_Macromol.Biosci_4_238 |
| Author(s) : Tsuge T , Saito Y , Kikkawa Y , Hiraishi T , Doi Y |
| Ref : Macromol Biosci , 4 :238 , 2004 |
|
Abstract :
A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB(-)4 harboring mutated PHA synthase genes (phaC(Ac)) from Aeromona caviae was investigated. The strain harboring wild-type phaC(Ac) gene produced a PHA copolymer consisting of (R)-3-hydroxybutyrate and (R)-3-hydroxyhexanoate [P(3HB-co-3HHx)] with 3.5 mol-% of 3HHx fraction from soybean oil. When the mutants of phaC(Ac) gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0-5.1 mol-%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes. |
| PubMedSearch : Tsuge_2004_Macromol.Biosci_4_238 |
| PubMedID: 15468213 |
| Gene_locus related to this paper: aerca-PHAC |
| Gene_locus | aerca-PHAC |
Tsuge T, Saito Y, Kikkawa Y, Hiraishi T, Doi Y (2004)
Biosynthesis and compositional regulation of poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in recombinant ralstonia eutropha expressing mutated polyhydroxyalkanoate synthase genes
Macromol Biosci
4 :238
Tsuge T, Saito Y, Kikkawa Y, Hiraishi T, Doi Y (2004)
Macromol Biosci
4 :238