| Title : Development of an acetylcholinesterase-choline oxidase based biosensor for acetylcholine determination - Tunc_2015_Artif.Cells.Nanomed.Biotechnol__1 |
| Author(s) : Tunc AT , Aynaci Koyuncu E , Arslan F |
| Ref : Artif Cells Nanomed Biotechnol , :1 , 2015 |
|
Abstract :
In this article, a novel amperometric acetylcholine (ACh) biosensor with immobilization of acetylcholinesterase-choline oxidase (AChE-ChO) on electrochemically polymerized polyaniline-polyvinylsulphonate (PANI-PVS) film has been successfully developed for the first time. AChE and ChO enzymes were immobilized on PANI-PVS film by cross-linking with using glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of ACh was carried out by oxidation of enzymatically produced H2O2 at +0.4 V versus Ag/AgCl. Optimum pH and temperature were found to be 8.0 and 60 degrees C, respectively. The linear part was in the region 1.0 x 10-7-6.0 x 10-7 M (R2 = 0.990). Storage stability and operation stability of the enzyme electrode were also studied. |
| PubMedSearch : Tunc_2015_Artif.Cells.Nanomed.Biotechnol__1 |
| PubMedID: 26367252 |
Tunc AT, Aynaci Koyuncu E, Arslan F (2015)
Development of an acetylcholinesterase-choline oxidase based biosensor for acetylcholine determination
Artif Cells Nanomed Biotechnol
:1
Tunc AT, Aynaci Koyuncu E, Arslan F (2015)
Artif Cells Nanomed Biotechnol
:1