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Uchida_2013_J.Pediatr.Surg_48_502

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Title : Clinical implications of CES2 RNA expression in neuroblastoma - Uchida_2013_J.Pediatr.Surg_48_502
Author(s) : Uchida K , Otake K , Tanaka K , Hashimoto K , Saigusa S , Matsushita K , Koike Y , Inoue M , Ueeda M , Okugawa Y , Inoue Y , Mohri Y , Kusunoki M
Ref : J Pediatr Surg , 48 :502 , 2013
Abstract :

BACKGROUND/PURPOSE: Human carboxylesterase 2 (CES2) is the key enzyme for metabolic activation of irinotecan (CPT-11). The aim was to evaluate the clinical implications of CES2 RNA expression in neuroblastoma cells.
METHODS: CES2 RNA expression was determined by real-time reverse transcription-polymerase chain reaction in five neuroblastoma cell lines and 42 clinical samples of untreated neuroblastoma. Sensitivity to CPT-11 was assessed by WST-8 colorimetric assays. Induction of apoptosis was evaluated by flow cytometry after CPT-11 exposure. Protein expression of CES2 was evaluated by Western blotting analysis. CES2 RNA expression in clinical samples was investigated for its associations with the clinicopathological characteristics.
RESULTS: CES2 RNA expression was observed in neuroblastoma cells, and its expression in neuroblastoma cell lines was positively correlated with sensitivity to CPT-11 and apoptosis after CPT-11 exposure in vitro. CES2 RNA expression was correlated with the protein levels of CES2 in vitro. CES2 RNA expression was significantly higher in patients with a characteristic related to advanced disease.
CONCLUSIONS: Our results suggest the potential of clinical application of CPT-11 in neuroblastoma treatment for patients with advanced disease.
PubMedSearch : Uchida_2013_J.Pediatr.Surg_48_502
PubMedID: 23480903

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Uchida K, Otake K, Tanaka K, Hashimoto K, Saigusa S, Matsushita K, Koike Y, Inoue M, Ueeda M, Okugawa Y, Inoue Y, Mohri Y, Kusunoki M (2013)
Clinical implications of CES2 RNA expression in neuroblastoma
J Pediatr Surg 48 :502

Uchida K, Otake K, Tanaka K, Hashimoto K, Saigusa S, Matsushita K, Koike Y, Inoue M, Ueeda M, Okugawa Y, Inoue Y, Mohri Y, Kusunoki M (2013)
J Pediatr Surg 48 :502